Sodium chloride sperm

This study evaluated sperm viability over time, after dilution and refrigerator storage of fresh semen extended in either synthetic cauda epididymal plasma CEP2 , or in a low sodium medium CJ2 supplemented with either AlbuMAX or egg yolk. Semen collected weekly for 4 weeks from 4 bulls and assigned within bulls, across treatments. A computer assisted sperm analysis CASA system was used for sperm evaluation. Particular emphasis was placed on sperm motility since it is the single most important sperm parameter influencing bull fertility. Semen handling and extender composition are crucial factors that affect spermatozoa after dilution [3] [4].
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Effect of Low-Sodium, Choline-Based Semen Diluent on Viability of Bovine Spermatozoa Stored at 4°C

In fish with external fertilization, two main start-up mechanisms of the path that blocks or activates the spermatozoan motility apparatus are known. The main factor managing the path is osmolality or potassium ion. In burbot from the European and North American population, contradictory findings regarding the factors influencing the onset of spermatozoa motility were reported. The objective of the current study was to determine the effect of potassium and osmolality on the spermatozoa activation of European burbot, Lota lota Actinopterygii, Gadiformes, Lotidae. Moreover, the influence of pH, as well as sodium ion concentrations on spermatozoa motility was investigated.
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Spermatozoal velocity and percentage of motility were analyzed objectively with the multiple exposure photography method before and after specimens from fertile and infertile men were diluted in their own seminal plasma or normal saline. No significant change in percentage of motility was found in samples diluted up to in both kinds of diluents. Compared with undiluted specimens, there was no delayed effect on spermatozoal motility when semen was diluted with saline after up to 4 hours' incubation time. Contrary to the findings in animal and human semen described by others, there was no deleterious effect on sperm motility with this kind and rate of dilution and duration of time. The assumption that the increase in sperm velocity caused by dilution is not excitatory but is due only to a decrease of seminal fluid viscosity and a reduced number of spermatozoa which interfere with sperm free movement is discussed.
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The sperm quality index SQI is a tool used to predict overall rooster semen quality, fertility, and hatchability. However, semen must be diluted before SQI analysis, and research has shown that the SQI is most predictive of fertility at lower semen dilutions. Therefore, the present study was undertaken to determine why the SQI is not as predictive of fertility at higher semen dilutions and whether semen diluent type alters the SQI, adenosine triphosphate ATP utilization, gas exchange, and ionic balance of broiler breeder sperm. Semen was diluted with saline, seminal plasma, or minimum essential medium MEM from 2- to fold.
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